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KMID : 1025520070490050585
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Journal of Animal Science and Technology
2007 Volume.49 No. 5 p.585 ~ p.592
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Effect of Glycerol Concentration, Freezing Rate and Thawing Rate on Semen Characteristics in PoongSan-dog
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Jj D. Y.
Rho Jung-Rae
Cho Sang-Rae
Kim C. K.
Pang Myung-Geol
Kim Bo-Suk
Yoon T. C.
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Abstract
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This research was carried out in order to establish the production technique for Poong-san dog’s frozen semen, by examining the semen characteristic and the volume of glycerol added to the dilution solution, thawing temperature and sperm motility and viability as well as the motility using CASA according to time variation.
Average semen volume was 5.9ml, sperm concentration 116.3×106 sperm/ml, total sperm number 789.3×106 sperm, motility 88.7±1.7% and viability 87.6±7.8%. When it was cryopreservation and thawed at different glycerol concentrated extender, it showed 52.7% motility and 57.7±10.3% viability at 7% glycerol, compared to other treatments. For semen cryogeny, at conditions of 5, 7cm and a height of 10cm for pre-cryogeny and maintaining the semen at 7cm from the surface of liquid nitrogen resulted in profitable motility and viability.
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KEYWORD
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PoongSan-dog, Semen characteristic, Glycerol, Freezing, Thawing
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